Vitreous Tap for Bacterial C/S (Aerobic) with Gram Stain

Specimen Required

Vitreous Tap

Fasting Required

Purpose of the Test

The Vitreous Tap for Bacterial C/S (Aerobic) with Gram Stain test is used to diagnose and identify the bacteria causing infections in the eye. The test is usually ordered by an ophthalmologist when there is a suspicion of an infection in the eye that has not responded to initial treatments.

When this test is required

Vitreous tap for bacterial C/S with Gram stain is typically requested by ophthalmologists or other eye specialists when there is suspicion of a bacterial infection in the eye, particularly if other treatments have not been effective or if the infection is severe. The procedure is usually performed under local anesthesia and takes only a few minutes to complete.

What the Test Detects

During the procedure, a sample of the clear gel-like substance called vitreous humor, which fills the inner chamber of the eye, is collected with a small needle. The sample is then sent to a laboratory for bacterial culture and sensitivity testing, which involves growing bacteria from the sample in a controlled environment and testing the bacteria’s sensitivity to different antibiotics.

Preparation for the Test

There is no specific preparation required for this test. However, the ophthalmologist may advise the patient to stop taking certain medications, such as blood thinners, before the procedure. It is important to follow the doctor’s instructions carefully to minimize the risk of complications.

Sample Requirements

To perform the test, a sample of the vitreous humor (the gel-like substance in the center of the eye) is obtained through a procedure called vitreous tap. The sample is then sent to a laboratory for culture and sensitivity testing, which can identify the type of bacteria causing the infection and determine which antibiotics will be effective in treating it.

Additional Notes

The sample obtained from the vitreous tap is cultured to identify the type of bacteria causing the infection, and a sensitivity test is performed to determine which antibiotics will be most effective in treating the infection. The gram stain is a preliminary test used to identify the type of bacteria present and helps to guide appropriate antibiotic therapy.

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