Serum Estradiol (E2)

Specimen Required

3-5cc Clotted Blood or Serum

Fasting Required

Purpose of the Test

Serum estradiol (E2) test is done to measure the levels of estradiol in the blood. It is done to evaluate fertility, diagnose and monitor hormonal imbalances, evaluate menstrual cycle irregularities, monitor hormone replacement therapy in postmenopausal women, and diagnose and monitor ovarian tumors and some other disorders.

When this test is required

The healthcare provider may order a serum estradiol (E2) test if a patient has symptoms of hormonal imbalance, such as infertility, menstrual irregularities, hot flashes, vaginal dryness, or mood swings. The test may also be ordered to monitor hormone replacement therapy, evaluate ovarian tumors, and diagnose or monitor other conditions.

What the Test Detects

The serum estradiol (E2) test measures the levels of estradiol in the blood. Estradiol is a type of estrogen hormone produced by the ovaries in females and in smaller amounts by the adrenal glands in both males and females. Estradiol plays a crucial role in the development of female reproductive organs and secondary sexual characteristics, regulates the menstrual cycle, and affects bone density and cholesterol metabolism.

Preparation for the Test

The healthcare provider may ask the patient to fast for a certain period before the test. They may also ask the patient to stop taking certain medications or supplements that can affect the estradiol levels in the blood.

Sample Requirements

A blood sample is collected from a vein in the arm for the serum estradiol test.

Additional Notes

The serum estradiol (E2) test is usually done along with other hormone tests such as follicle-stimulating hormone (FSH), luteinizing hormone (LH), and progesterone to get a comprehensive evaluation of the hormonal status of the patient. The test results should always be interpreted in the context of the patient’s medical history, symptoms, and other relevant factors.

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