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Semen for Analysis

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SEMEN

Fasting Required

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Purpose of the Test

To investigate to see if there is a problem with your fertility. This is often performed if you are in a heterosexual relationship and your partner is having trouble becoming pregnant.It is also necessary after a vasectomy operation to ensure that the sterilisation operation was successful and that you are no longer fertile. This test is called a post-vasectomy semen analysis (PVSA).

When this test is required

There are two main reasons for semen analysis.Following vasectomy: Semen analysis is recommended following a vasectomy to make sure that the sterilisation operation has been successful.Testing for infertility: Semen analysis is performed when your doctor thinks that you might have a fertility problem. About one in six couples experience problems conceiving. Male infertility is on the increase and male factors are implicated about 50% of the time. Male infertility has many causes and some of these can be treated successfully. If male factors are the cause, analysis of the semen is necessary to determine what is preventing fertility and to evaluate whether assisted reproductive technology (e.g. IVF) would be helpful. If sperm are suboptimal, a form of IVF called ICSI (intracytoplasmic sperm injection) can be used, whereby a single sperm is carefully injected directly into the woman’s egg to cause fertilisation. When a semen analysis is abnormal, your doctor may ask for one or more repeat tests.

What the Test Detects

A semen analysis measures the quantity and quality of the fluid released from the penis during ejaculation. It evaluates both the liquid portion, called semen, and the microscopic moving cells, called spermatozoa (sperm). Semen is the turbid, whitish liquid that contains sperm and the products from several glands. It is normally fairly thick after ejaculation and then becomes thinner within ten to thirty minutes. Sperm are the…A semen analysis measures the quantity and quality of the fluid released from the penis during ejaculation. It evaluates both the liquid portion, called semen, and the microscopic moving cells, called spermatozoa (sperm). Semen is the turbid, whitish liquid that contains sperm and the products from several glands. It is normally fairly thick after ejaculation and then becomes thinner within ten to thirty minutes. Sperm are the reproductive cells in semen that have a head and a tail. Each sperm contains one copy of each chromosome (all of the male’s genes). Sperm should be progressively motile (can move forward independently) and this allows them to travel to and fuse with the female’s egg, resulting in fertilisation. In each semen sample, there should be millions of sperm and varying amounts of other substances that support fertilisation. A typical semen analysis could measures:Volume of semen Consistency (thickness) of the semen Sperm concentration Total number of sperm Sperm motility (the percentage that are able to move, as well as how vigorously and progressively the sperm move) Number of normal-shaped and not normal-shaped (defective) sperm Coagulation and liquefaction Fructose (a sugar in semen) pH (acidity) Number of immature sperm Number of white blood cells (cells that indicate infection) Additional tests may be performed if semen is abnormal, such as a test for antisperm antibodies. If assisted reproductive technology is contemplated, e.g. in vitro fertilisation (IVF), sperm function tests may also be performed. How is the sample collected for testing? Most laboratories require samples to be collected at the hospital as the semen needs to be examined within one hour after ejaculation for a fertility semen analysis. Semen is collected in a dedicated private area with a locked door. The man masturbates and collects the semen in a sterile specimen container. Some men, for religious or other reasons, might want to collect semen during the act of intercourse, using a condom. If this is the case, the man should ask doctor to provide a silastic condom which is specifically made to be non-toxic to sperm. Do not use standard condoms as these are usually spermicidal. Sperm are very temperature sensitive. If the sample is collected at home, the sample should be kept next to the body during transportation. It should not be left at room temperature and should not be refrigerated or heated. The sample must be delivered to the lab within one hour for analysis. Is any test preparation needed to ensure the quality of the sample? In order for the test to be reliable, you need to abstain from masturbation or sex for two to seven days before the sample is collected. You should follow the instructions given to you by the laboratory or your doctor. You should not collect the sample via intercourse or oral stimulation, as this can contaminate the sample. The penis and genitals should be thoroughly cleaned, rinsed and dried before sample production. No lubricants should be used, as these can be toxic to sperm. See MoreSee Less

Preparation for the Test

In order for the test to be reliable, you may need to abstain from masturbation or sex for two to seven days before the sample is collected. You should follow the instructions given to you by the laboratory or your doctor.

Sample Requirements

A semen sample collected in a sterile container provided by the laboratory or doctor. It is important that the specimen container has been tested to ensure it is not toxic to sperm.

Additional Notes

Common causes of male infertility include reduced testicular function (failure of the testicles to produce enough sperm) and obstruction of the tubes that carry semen to the penis.When a doctor is evaluating a man’s fertility, each aspect of the semen analysis is considered, as well as the findings as a whole. Each part of the semen analysis either contributes to fertility or lessens it, but the results do not necessarily predict the eventual outcome. Couples with poor results may still conceive, with or without assistance, and those with apparently good results may experience difficulties.Several factors can affect the sperm count and other semen analysis values. A man may have a lower sperm count if he has damage to the testicles, has gone through radiation treatment of his testicles, or has had exposure to certain drugs (such as azathioprine, cimetidine, and some cancer chemotherapy drugs). A man with a higher level of oestrogens may have a lower sperm count.