Pleural Fluid for Bacterial C/S (Aerobic) with Gram Stain

Specimen Required

FLUID (All Body Fluid)

Fasting Required

Purpose of the Test

The Pleural Fluid for Bacterial C/S (Aerobic) with Gram Stain test is ordered to detect and identify bacterial infection in the pleural fluid, which is the fluid that surrounds the lungs and helps them move smoothly during breathing. This test helps in the diagnosis of pleural effusion, which is the accumulation of excess fluid in the pleural space, often caused by infections, such as pneumonia or tuberculosis.

When this test is required

The Pleural Fluid for Bacterial C/S (Aerobic) with Gram Stain test is requested when a person has symptoms of pleural effusion, such as chest pain, shortness of breath, or cough, and the healthcare provider suspects a bacterial infection as the underlying cause. The test may also be ordered if a person has a fever or other signs of infection, or if they have a history of recent respiratory illness or tuberculosis.

What the Test Detects

The Pleural Fluid for Bacterial C/S (Aerobic) with Gram Stain test involves two parts: 1) Gram stain: This test involves staining the pleural fluid with a special dye that can differentiate between different types of bacteria based on their cell wall properties. The stained fluid is then examined under a microscope to look for the presence of bacteria and their characteristics. 2) Bacterial culture and sensitivity (C/S): This test involves placing a small amount of pleural fluid onto a culture medium that supports the growth of bacteria. The medium is then incubated for several days to allow the bacteria to grow. Once the bacteria have grown, they can be identified using various biochemical tests and their sensitivity to different antibiotics can be determined.

Preparation for the Test

No special preparation is required for the Pleural Fluid for Bacterial C/S (Aerobic) with Gram Stain test.

Sample Requirements

The test requires a sample of pleural fluid, which is obtained by a healthcare provider using a needle inserted into the pleural space between the lungs and the chest wall. The fluid is then collected in a sterile container and sent to the laboratory for testing.

Additional Notes

The Pleural Fluid for Bacterial C/S (Aerobic) with Gram Stain test is just one of several tests that may be ordered to diagnose pleural effusion. Other tests, such as viral cultures, acid-fast bacilli (AFB) stains, and cytology, may also be necessary depending on the suspected underlying cause.

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