Peritoneal Fluid for AFB Smear/ZN Stain

Specimen Required

Pancreatic fluid

Fasting Required

Purpose of the Peritoneal Fluid for AFB Smear/ZN Stain Test

The Peritoneal Fluid for AFB Smear/ZN Stain test is performed to detect the presence of acid-fast bacilli (AFB) in the peritoneal fluid sample. The test helps in the diagnosis of tuberculosis or other mycobacterial infections in the peritoneum, which is the lining of the abdominal cavity.

When this Peritoneal Fluid for AFB Smear/ZN Stain test is required

A healthcare provider may order a Peritoneal Fluid for AFB Smear/ZN Stain test if a person has symptoms of peritoneal tuberculosis or if there is a suspicion of a mycobacterial infection in the peritoneum. The test may also be ordered if a person has a positive tuberculin skin test or a history of exposure to tuberculosis.

What the Peritoneal Fluid for AFB Smear/ZN Stain Test Detects

The Peritoneal Fluid for AFB Smear/ZN Stain test is used to detect the presence of acid-fast bacilli (AFB) in the peritoneal fluid sample. AFB are a type of bacteria that have a waxy coating on their cell wall, which makes them resistant to many standard staining techniques. However, they can be visualized using the Ziehl-Neelsen (ZN) stain, which is a specific staining technique that targets the waxy coat of the AFB.

Preparation for the Peritoneal Fluid for AFB Smear/ZN Stain Test

There is no specific preparation required for the test. However, a person should inform the healthcare provider about any medications they are taking, as some drugs may interfere with the test results. In summary, a Peritoneal Fluid for AFB Smear/ZN Stain test is ordered to diagnose tuberculosis or other mycobacterial infections in the peritoneum. It is performed on a sample of peritoneal fluid collected using a needle and syringe, and no specific preparation is needed for the test.

Sample Requirements

To perform the test, a sample of peritoneal fluid is collected using a needle and syringe, under sterile conditions. The collected sample is then smeared on a glass slide and stained using the Ziehl-Neelsen (ZN) stain, which helps to visualize the AFB under a microscope.

Additional Notes

It’s important to note that a negative result on the Peritoneal Fluid for AFB Smear/ZN Stain test does not necessarily rule out the possibility of peritoneal tuberculosis or other mycobacterial infections. Further testing, such as a culture or molecular tests, may be required to confirm or rule out the diagnosis.

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