Pancreatic Fluid for AFB Smear/ZN Stain

Specimen Required

Cystic Fluid

Fasting Required

Purpose of the Test

The Pancreatic Fluid for AFB Smear/ZN Stain test is ordered to detect the presence of acid-fast bacilli (AFB) in the pancreatic fluid, which may indicate an infection with Mycobacterium tuberculosis or other related species. This test is primarily used to diagnose tuberculosis (TB) or other mycobacterial infections affecting the pancreas.

When this test is required

The test is typically requested when a patient presents with symptoms suggestive of TB or other mycobacterial infections affecting the pancreas, such as abdominal pain, nausea, vomiting, diarrhea, and fever. The test may also be ordered if the patient has a history of TB or exposure to someone with TB.

What the Test Detects

The Pancreatic Fluid for AFB Smear/ZN Stain test is used to detect the presence of acid-fast bacilli (AFB) in the pancreatic fluid, which may indicate an infection with Mycobacterium tuberculosis or other related species. The test involves staining the sample of pancreatic fluid with a special dye called Ziehl-Neelsen (ZN) stain, which can identify the presence of AFB under a microscope.

Preparation for the Test

There is no specific preparation needed for this test, but patients may need to fast for several hours prior to the procedure, and should inform their healthcare provider of any medications they are taking, as certain medications may need to be temporarily discontinued before the test. It is also important to inform the healthcare provider of any allergies or medical conditions before undergoing the procedure.

Sample Requirements

A sample of pancreatic fluid is required for this test. The sample is collected using a thin, flexible tube called an endoscope, which is passed through the mouth and into the small intestine. Once in the small intestine, the endoscope is guided to the pancreas, where a small amount of fluid is collected using a needle.

Additional Notes

It is important to note that a negative result does not rule out the possibility of TB or other mycobacterial infections affecting the pancreas. In some cases, the bacteria may not be present in the sample tested, or the sample may not have been collected properly. Additional tests, such as culture or PCR, may be necessary to confirm a diagnosis.

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