MRSA PCR (Methicillin Resistant Staphylococcus Aureus)

Specimen Required

Fasting Required

Purpose of the Test

To detect MRSA (Meticillin resistant Staphylococcus Aureus) carrier status

When this test is required

MRSA screening tests may be requested when a doctor, hospital, or researcher wants to evaluate potential MRSA colonisation in an individual, their family members or a group of people in the community as the source of a MRSA infection. Specific populations that have close contact such as residents of a nursing home or health care workers may be tested for MRSA carrier status when an increased number of infections occur within their close group. MRSA screening may also be requested on a person who has been treated for an MRSA infection or for MRSA colonisation to determine whether MRSA is still present on the skin or wound site. MRSA screening is also carried out before hospital elective surgery admissions and on emergency hospital admissions. Screening identifies colonised or infected individuals who can then be managed and to reduce the spread of MRSA to others.

What the Test Detects

This test detects the presence of Meticillin Resistant Staphylococcus aureus (MRSA) and sometimes evaluates the genetic characteristics of the strain.MRSA are strains of Staphylococcus aureus, or “staph,” bacteria that have become resistant to some of the antibiotics commonly used to treat these type of infections, (the beta-lactam group of antibiotics) which include the penicillins, meticillin and cephalosporins. Standard courses of antibiotics may be adequate to treat regular “staph” infections but often lead to treatment failure in patients with MRSA. Stains of MRSA were first identified in the early 1960’s and MRSA outbreaks have been a problem in confined populations such as hospitals, prisons, and nursing homes ever since. MRSA strains have caused a significant number of severe skin, lung, bone, and heart-related infections that have proven difficult to treat and in some cases proven fatal. All hospitals have implemented measures in an attempt to eradicate MRSA and to control the spread of MRSA from person to person. This has been a challenge as “staph” is a common bacterium that colonises the skin and in the nose of about 20-40% of the population. In the past, only about 0.8 % of the colonising “staph” were MRSA, but in the last decade this has risen to 1-3%, and studies of select populations have shown MRSA colonisation rates as high as 22% in care home residents.There have been outbreaks of MRSA outside the hospital setting and in the last few years the number of cases have greatly increased. Therefore raising concern among doctors and other healthcare workers. In the community, MRSA is causing infections in young previously healthy people with no apparent risk factors of infection. Studies of these cases have shown that the bacteria are being spread in the community by MRSA colonised or infected people through close contact (such as sports or a day care) and through contact with contaminated objects (such as sports equipment, shared towels or razors) Often the infection it causes will be a long-lasting skin infection.Studies have also shown that the community acquired strains of MRSA were frequently genetically different from those found in the hospital setting (indicating that they developed separately). They were resistant to antibiotics routinely prescribed to treat skin infections and in some cases have proven to be especially harmful, producing toxins and causing an invasive infection. These strains of MRSA are now being found in hospitals as well, with infected and/or colonised patients and healthcare workers bringing them into this setting.

Preparation for the Test

None

Sample Requirements

Swabs of nose and throat. Occasionally swab of wound infection site, groin, or skin lesion swab

Additional Notes

A sampling of positive MRSA tests may be subjected to further testing to help investigate the spread of MRSA within a community or region but are not often used in the treatment of an individual patient. These include pulsed-field gel electrophoresis (PFGE) which can identify the type and subtype of S. aureus strains and DNA testing, which can be used to look at the genetic material of the bacteria and detect the presence or absence of the mecA gene, which confers resistance to meticillin, and flucloxacillin antibiotics.DNA testing can also be performed to detect the presence of the Panton-Valentine leukocidin (PVL) gene. This gene is associated with the production of a toxin that can greatly increase the complications associated with MRSA infections and can occasionally prove fatal. People positive for PVL require prolonged treatment with antibiotics.Public awareness of MRSA and measures to control its spread are growing. With the importance of good hand hygiene before and after direct patient contact or patients surroundings (bed, table or equipment). Doctors are being encouraged to request MRSA screening on their patients with skin infections, in cases where they suspect a MRSA carrier and prior to hospital admission or elective surgery. Standard courses of antibiotics may be adequate to treat regular “staph” infections but often lead to treatment failure in patients with MRSA. Suppression of MRSA carriage can be treated by the use of 2% mupirocin nasal cream and 4% chlorohexidine gluconate shampoo/body wash.

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