DNA Extraction from FFPE for Research

Specimen Required

Fasting Required

Purpose of the Test

DNA extraction from FFPE tissue can be useful in research for several reasons, including:Studying genetic variations in cancer or other diseasesInvestigating the effectiveness of new therapies or treatmentsIdentifying genetic markers for disease diagnosis or prognosisStudying gene expression patterns in tissue samples

When this test is required

The decision to request DNA extraction from FFPE tissue samples for research purposes is typically made by researchers or healthcare providers. The timing of the test depends on the research question being addressed and the availability of FFPE tissue samples.

What the Test Detects

The DNA extracted from FFPE tissue can be used to study genetic variations in cancer or other diseases, investigate the effectiveness of new therapies or treatments, identify genetic markers for disease diagnosis or prognosis, and study gene expression patterns in tissue samples.

Preparation for the Test

The preparation of FFPE tissue samples for DNA extraction typically involves several steps, including deparaffinization, rehydration, and DNA extraction. The specific protocol used for DNA extraction may vary depending on the type of tissue sample and the research question being addressed.

Sample Requirements

The sample required for DNA extraction from FFPE tissue is typically a small piece of tissue that has been preserved in formalin and embedded in paraffin. The tissue can be obtained from a biopsy or surgical specimen.

Additional Notes

DNA extraction from FFPE tissue samples can be challenging due to the effects of formalin fixation and paraffin embedding on the quality and quantity of DNA. Therefore, it is important to use appropriate techniques and quality control measures to ensure the accuracy and reliability of the results. Additionally, it is important to obtain informed consent from patients or their families before using FFPE tissue samples for research purposes.

Selected Tests