Bronchial Washings for Gram Stain

Specimen Required

BRONCHIAL WASHING

Fasting Required

Purpose of the Test

Bronchial washings for Gram stain is a diagnostic test used to identify bacteria in the lungs that may be causing respiratory infections. This test is particularly helpful in identifying the cause of pneumonia, bronchitis, and other lung infections.

When this test is required

A healthcare provider may request a bronchial washing for Gram stain if a patient has symptoms of a respiratory infection, such as fever, cough, shortness of breath, and chest pain. The test may also be ordered if a patient has been diagnosed with pneumonia or another lung infection and the healthcare provider wants to identify the specific bacteria causing the infection.

What the Test Detects

Bronchial washings for Gram stain is a laboratory test that is used to identify bacterial infections in the lungs. This test is performed on a sample of fluid that is collected by washing the bronchial lining with saline solution during a bronchoscopy procedure.

Preparation for the Test

No special preparation is needed for a bronchial washing for Gram stain. However, your healthcare provider may give you instructions on fasting or avoiding certain medications before the procedure. You should also inform your healthcare provider if you are allergic to any medications or if you have a bleeding disorder.

Sample Requirements

To perform the test, a healthcare provider will insert a bronchoscope, a flexible tube with a camera, through your mouth or nose and down into your lungs. They will then wash the lining of your lungs with saline solution to collect a sample of cells and bacteria. The sample will be stained with crystal violet and iodine and examined under a microscope to identify the presence of bacteria.

Additional Notes

It is important to note that the bronchial washing for Gram stain is not a definitive test for identifying all types of bacteria. Some bacteria may not be visible under a microscope, and further testing may be needed to identify the specific type of bacteria causing the infection.

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